Coding

Part:BBa_K944004:Design

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


cioB gene related to cyanide insensitivity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 445
    Illegal NgoMIV site found at 852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172


Design Notes

The cioB naturaly had a Pst1 restriction site in its sequence so we did a silent mutation for no longer to be this restriction site and proceed with the standard protocol 10.


Source

This part came from the Pseudomonas aeruginosa genomic sequence and synthesized by IDT Technologies.

References